How to choose the right CRISPR-Cas9 delivery method?

"Everyone is a writer" program aims to promote the sharing of knowledge and learning together. There are some answers about the CRISPR/Cas9 from this campaign.

Merin Kurian: Currently, there are several methods for delivering CRISPR systems in vivo and in vitro. Both delivery vectors and physical methods are widely implemented for the efficient delivery of CRISPR/Cas9-mediated genome editing. Delivery vectors such as viral and non-viral vectors can accommodate mRNA or plasmid expressing the nucleases to target cells or tissues. Learn More

Ana Luiza Dias Abdo Agamme: When planning a CRISPR Cas9 experiment, you need to choose a method that works well for your target cell type and minimizes cell toxicity. Delivery in the form of Cas9-sgRNA ribonucleoprotein complexes (RNPs): can be done by electroporation or vesicles. Learn More

Elizabeth Gabriela Macedo Flores: Just as important as good sgRNA design is the choice of a conducive to the introduction of the genome editing tool based on cell type that you want to modify in order to successfully achieve the change in the target gene. At In this sense, several formats for introducing nuclease are available, such as in the form of DNA, mRNA or protein with DNA transfection being the most (Yamamoto 2015). The following are the different approaches that are addressed for the transfection of nuclease and sgRNA either following biochemical, physical, viral or protein fusion methods. Learn More

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