Michael O. Agaphonov

The major directions of research of Dr. Agaphonov’s group involve the study of functioning of secretory organelles using the yeasts Ogataea polymorpha and O. parapolymorpha as model organisms. One of the projects involves study of the role of the secretory-pathway of protein glycosylation in the regulation of cell physiology. In contrast to the well-studied attachment of glycoside chains to polypeptides in the endoplasmic reticulum, the role of sugar moieties attached in the Golgi apparatus remains insufficiently studied, and that drives the study of manifestations of Golgi glycosylation defects being a major interest of Agaphonov’s group. Beside this, the group works on the development of different approaches for the creation of O. polymorpha and O. parapolymorpha strains producing recombinant proteins for industrial applications.

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Group of Genetic Engineering of Lower Eukaryotes, The Russian Academy of Sciences

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Protein Glycosylation Pathways 0 O. polymorpha-based Recombinant Protein Production 0 Golgi glycosylation 0

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  1. Karginov AV, Fokina AV, Kang HA, Kalebina TS, Sabirzyanova TA, Ter-Avanesyan MD, Agaphonov MO. Dissection of differential vanadate sensitivity in two Ogataea species links protein glycosylation and phosphate transport regulation. Sci Rep. 2018, 8:16428. doi: 10.1038/s41598-018-34888-5.

  2. Agaphonov MO. Improvement of a yeast self-excising integrative vector by prevention of expression leakage of the intronated Cre recombinase gene during plasmid maintenance in Escherichia coli. FEMS Microbiol Lett. 2017 364(22).doi: 10.1093/femsle/fnx222.

  3. Karginov A, Agaphonov M. A simple enrichment procedure improves detection of membrane proteins by immunoblotting. Biotechniques. 2016, 61:260-261.

  4. Fokina AV, Chechenova MB, Karginov AV, Ter-Avanesyan MD, Agaphonov MO. Genetic Evidence for the Role of the Vacuole in Supplying Secretory Organelles with Ca2+ in Hansenula polymorpha. PLoS One. 2015, 10:e0145915. doi:10.1371/journal.pone.0145915.

  5. Moon HY, Cheon SA, Kim H, Agaphonov MO, Kwon O, Oh DB, Kim JY, Kang HA. Hansenula polymorpha Hac1p Is Critical to Protein N-Glycosylation Activity Modulation, as Revealed by Functional and Transcriptomic Analyses. Appl Environ Microbiol. 2015, 81:6982-93. doi: 10.1128/AEM.01440-15.


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