Duhee Bang

Dunhee Bang became full Professor at department of Chemistry of Yonsei University, Seoul, Korea in 2019. He completed a Ph.D. in Prof.Stephen Kent’s group at the University of Chicago, where he later received postdoctoral training. Prof. Bang’s laboratory is devoted to the creation of novel bio-technologies, as they are exploiting experimental and computational perspectives of biology, focusing on the area of genetics. The major research themes of his lab encompass the development of next generation sequencing technologies for genetic materials, as well as the incorporation of CRISPR/Cas9 systems into study in genotype-phenotype relations, and for the therapeutic applications. The lab has been contributing to the improvement of current sequencing techniques – they came out with an oligonucleotide “barcoding’ method to allow a cost-effective single-cell RNA sequencing in multiple samples (Shin et al., 2019). In a recent project, they combined CRISPR RNA-guided deaminase and scRNA-seq technology to develop a platform for introducing mutations in multiple genes and assessing the mutation-associated signatures, which is used in (Jun et al., 2020). Bang’s group is also interested in Synthetic Biology, focused on developing innovative and disruptive technologies.

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Dunheebang Lab at Yonsei University

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Single-Cell Sequencing Technologies 0 CRISPR/Cas9 0 Genome Engineering 0 Synthetic Biology 0

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  1. Park M, Lee D, Bang D, Lee JH. MAPS-seq: magnetic bead-assisted parallel single-cell gene expression profiling. Exp Mol Med. 2020;52(5):804-814. doi:10.1038/s12276-020-0433-x

  2. Kang JK, Heo S, Kim HP, et al. Liquid biopsy-based tumor profiling for metastatic colorectal cancer patients with ultra-deep targeted sequencing. PLoS One. 2020;15(5):e0232754. Published 2020 May 7. doi:10.1371/journal.pone.0232754

  3. Lim H, Jun S, Park M, et al. Multiplex Generation, Tracking, and Functional Screening of Substitution Mutants Using a CRISPR/Retron System. ACS Synth Biol. 2020;9(5):1003-1009. doi:10.1021/acssynbio.0c00002

  4. Jun S, Lim H, Chun H, Lee JH, Bang D. Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells. Commun Biol. 2020;3(1):154. Published 2020 Apr 2. doi:10.1038/s42003-020-0888-2

  5. Shin D, Lee W, Lee JH, Bang D. Multiplexed single-cell RNA-seq via transient barcoding for simultaneous expression profiling of various drug perturbations. Sci Adv. 2019;5(5):eaav2249. Published 2019 May 15. doi:10.1126/sciadv.aav2249

  6. Hwang B, Lee W, Yum SY, et al. Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements. Nat Commun. 2019;10(1):1234. Published 2019 Mar 15. doi:10.1038/s41467-019-09203-z


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