Christopher Hellen

Christopher Hellen is a Professor in the department of Cell Biology of SUNY Downstate Medical Center. Research in Dr. Chris Hellen's laboratory is concerned with how the cellular apparatus for protein synthesis in higher eukaryotes is usurped by viruses. Translation of most eukaryotic mRNAs is end-dependent, and proceeds by a mechanism in which a 43S complex comprising eIF1, eIF1A, eIF3, an [eIF2-initiator tRNA-GTP] complex and a 40S ribosomal subunit binds to the capped 5'-end of a mRNA and then scans the 5' nontranslated region to locate the initiation codon, where it forms a 48S complex. Ribosomal attachment is mediated by the cap-binding complex eIF4F, which consist of eIF4E, eIF4A and eIF4G subunits. eIF5 and eIF5B promote joining of a 60S ribosomal subunit to the 48S complex to form an 80S complex that is competent to begin protein synthesis.

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SUNY Downstate Health Sciences University, Department of Cell Biology

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  1. Pestova TV and CUT Hellen. 2003. Factor requirements for translation elongation after initiation at the A site on the Cricket paralysis virus internal ribosomal entry site. Genes and Development 17: 181-186.

  2. Kolupaeva VG, IB Lomakin, TV Pestova, CUT Hellen. 2003. Eukaryotic initiation factors eIF4G and eIF4A mediate conformational changes downstream of the initiation codon of the Encephalomyocarditis virus internal ribosomal entry site. Mol. Cell. Biol. 23: 687-98.

  3. Hellen CUT & PS Sarnow. 2001. Internal ribosomal entry sites in eukaryotic mRNA molecules. Genes and Development 15:1593-612.

  4. Pestova TV, VG Kolupaeva, IB Lomakin, EV Pilipenko, IN Shatsky, VI Agol and CUT Hellen. 2001. Molecular events in initiation of translation in eukaryotes. Proc. Natl. Acad. Sci. USA 98:7029-36

  5. Marcotrigiano J, IB Lomakin, N Sonenberg, TV Pestova, CUT Hellen & SK Burley. 2001. A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery. Molecular Cell 7: 193-203.

  6. Pilipenko EV, TV Pestova, VG Kolupaeva, EV Khitrina, AN Poperechnaya, VI Agol & CUT Hellen. 2000. A cell cycle-dependent protein serves as a template-specific translation initiation factor. Genes & Development 14: 2028-2045.

  7. Wilson JE, Pestova TV, Hellen CUT & Sarnow P. 2000. Initiation of protein synthesis from the A-site of the ribosome. Cell 102: 511-520.

  8. Kolupaeva VG, TV Pestova & CUT Hellen. 2000. An enzymatic footprinting enzymatic footprinting analysis of the interaction of 40S ribosomal subunits the internal ribosomal entry site of hepatitis C virus. Journal of Virology 74: 6242-6250.

  9. Kolupaeva VG, TV Pestova & CUT Hellen. 2000. Ribosomal binding to the internal ribosomal entry site of classical swine fever virus. RNA 6: 1791-1807.


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