What is AmpR promoter?

AmpR, the transcriptional regulator for the Citrobacter freundii ampC β‐lactamase gene, belongs to the LysR family of transcriptional regulators that typically autorepress their own expression (Ryuichi et.al, 2017). AmpR promoter is the promoter for ampicillin resistance. It is a weak promoter. Any gene can also be cloned under this promoter and expressed in bacteria like E. Coli.

There is a pCutamp plasmid used for plasmid-curing in Escherichia coli by targeting the AmpR promoter. The information as follows:


Plasmid Name: pCutamp

Bacterial Resistance: Apramycin

Plasmid Size (bp): 14593   ­­Sequence by Depositor

Description: Plasmid-curing in Escherichia coli by targeting the AmpR promoter

Publication: Zhang, Y., Sun, X., Wang, Q., Xu, J., Dong, F., Yang, S., ... & Zhang, J. (2020). Multicopy chromosomal integration using CRISPR-associated transposases. ACS synthetic biology.

There are also some parts named AmpR promoter on MolecularCloud. The information as follows:

1. Part Name: AmpR promoter 

    Cat. No.: MI_0000541

    Description: Promoter for ampicillin resistance; Medium Transcription

    Size (bp): 105

2. Part Name: AmpRpromoter 

    Cat. No.: MI_0000542

    Description: Promoter for ampicillin resistance; Medium Transcription

    Size (bp): 92

3. Part Name: AmpRpromoter 

    Cat. No.: MI_0000543

    Description: Promoter for ampicillin resistance; Medium Transcription

    Size (bp): 72

4. Part Name: AmpR promoter 

    Cat. No.: MI_0000544

    Description: Promoter for ampicillin resistance

    Size (bp): 103

5. Part Name: AmpR promoter 

    Cat. No.: MI_0000545

    Description: Promoter for ampicilin resistance

    Size (bp): 35

Find more plasmids and parts   here.


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