The Introduction of NeoR and KanR

Amy Johnson 22:09:50 10/21/2020

The protein name of KanR is Aminoglycoside 3'-phosphotransferase. It is from Brucella abortus. The expression of KanR is under the control of a prokaryotic promoter not active in eukaryotes. NeoR’s protein name is also Aminoglycoside 3'-phosphotransferase. But it is from Salmonella enterica subsp. enterica serovar Heidelberg and active in eukaryotes (UniProt).

Here is a part named NeoR – KanR on MolecularCloud. The information as follows:

Part Name: NeoR - KanR

Description: Aminoglycoside phosphotransferase from Tn5 confers resistance to kanamycin in bacteria, or neomycin and G418 (Geneticin(R)) in eukarytoes.

Size (bp): 795

Sequence:

ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGC

TATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCC

GGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGC

CCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGC

GTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGC

TATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAG

AAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACC

TGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGA

AGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAG

CCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGT

GACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGG

ATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGG

CTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTG

CTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGAC

GAGTTCTTCTGA

Find more plasmids and parts here.

3 Reply

Akash Kumar

08:45:45 01/11/2021

Hi Amy, Thanks for this information. Can you help me with what is the sequence of a native (or generally used) KanR promoters. I couldn't find one before KanR gene in my prokaryotic plasmid. Thanks

Akash Kumar reply Amy Johnson

11:35:32 02/27/2021

Thanks for the Reply Amy. The two sequences are of KanR gene. Probably, I couldn't articulate the question clearly last time. Actually I do have the 'gene' sequence, and need only the 'promoter' sequence. Kindly share if you have some info. Thanks

Amy Johnson reply Akash Kumar

03:33:10 02/02/2021

The sequence of generally used KanR promoter is MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF* or MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*

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