MolecularCloud October Newsletter: Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response

The CRISPR-Cas9 system has been adopted as a tool to manipulate the genomes of multiple living organisms, accelerating the pace of fundamental research and enabling clinical and agricultural breakthroughs. Besides, a newer type of genome editing approaches, named base-editor (BE) techniques, was recently developed. This kind of tool utilizes components from CRISPR systems together with other enzymes to directly install point mutations into cellular DNA or RNA. Nevertheless, base editors deriving from Cas9 nickase can cause unwanted DNA damage response (DDR). 

Dr. Jia Chen’s team published the paper “Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response” in Cell Reports on June 2, 2020 (Wang Xiao, et al., 2020 ). In this article, the researchers demonstrated that the original version of catalytically dead Cas12a (dCas12a)-conjugated BEs induce a basal level of DNA breaks and minimally activate DDR proteins, including H2AX, ATM, ATR, and p53. They went on further to develop a dCas12a-based BEACON (base editing induced by human APOBEC3A and Cas12a without DNA break) system through fusing dCas12a with engineered human apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A), and successfully applied it for both in vitro and in vivo editing. The BEACON system achieves efficient and specific base editing without generating DNA breaks or triggering DDR cascades, which is essential for its broad applications in mammalian cells and in clinics. 



Plasmids from this article on MolecularCloud
Plasmids (pCmv-BEACON1 and pCmv-BEACON2) of this article have been deposited on MolecularCloud.

Cat. No.

Plasmid Name

Description

MC_0101187
pCmv-BEACON1
Mammalian expression of BEACON1 human cells

MC_0101188

pCmv-BEACON2

Mammalian expression of BEACON2 human cells






Limited time offer
The two plasmids have also been listed in the campaign "Facilitate Gene Editing Research by Freeing the Plasmids". Click the anchor text  to learn more.

Previous newsletters
MolecularCloud September Newsletter: Winners Announced of 2020 Distinguished Research Awards
MolecularCloud August Newsletter: Glycosylase base editors enable C-to-A and C-to-G base changes
MolecularCloud July Newsletter: Multicopy Chromosomal Integration Using CRISPR-Associated Transposases
Related Campaign
Let's talk about genome editing-Everyone is a Writer


9 Reply

It seems to be a good news for well edition of genes. I want to have a try of both plasmids if possible.


Good news, I hope to be very honored to use it


This is good news, which means that gene editing technology has taken a big step forward in clinical application.


Great plasmids! Can you guys go to the website below to "favorite" them and make them free? I need them! https://www.molecularcloud.org/free-plasmid-promotion.html


It is a really interesting tool for edition, looking forward to try


As someone involved in DNA damage repair pathways, I can say this is a pair of very good tools to have.


I would like to give them a try as well, especially if we could get our hands on a free plasmid! Best regards from Belgium.


I want to both Plasmids (pCmv-BEACON1 and pCmv-BEACON2) of this article


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