Malaria is a human infectious disease often transmitted by mosquitoes. Researches are now altering a mosquito's gut genes using CRISPR-Cas9 gene-editing technology to reduce its ability to spread the disease. As long as the modified gene is passed to the next generation of their ...Learn More

Introduction of the prime editing vectors developed in Chen Lab Recently, a novel, universal, and precise genome-editing technology named prime editing was developed (Anzalone et al. Nature. 2019, 576: 149–57). We generated a set of pGreen3 binary vectors for prime editing in ...Learn More

The pCas/pTargetF system is one of the most popular genomic editing systems in E. coli. According to data from MolecularCloud, Addgene and our laboratory, there were 723 and 615 usage records in total for pCas and pTargetF respectively. However, few users pointed out that this ...Learn More

The CRISPR/Cas9 gene-editing tool kit has facilitated gene knockins above any other strategy developed to date. Still, improving efficiency and specificity remain challenging aspects of CRISPR/Cas9 directed gene insertions. In a continued effort to enhance knockin efficiency, ...Learn More

CRISPR news took center stage last year, with the 2020 Nobel Prize in Chemistry awarded to pioneers in the field, Dr. Jennifer A. Doudna and Dr. Emmanuelle Charpentier. The Nobel Prize win was the icing on the cake. Overall advances based on CRISPR editing tools were impressive in ...Learn More