MspA-H plasmid from Shuo Huang

Sequence by GenScript Sequence by Depositor

MspA-H

Cat. No.:

MC_0068402

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$65

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Keywords	Expression
Vector Backbone	Pet-30a(+)
Source	Member Deposit
Depositor	Shuo Huang
Organization	Nanjing University
Description	The plasmid MspA-H is designed for the preparation of a mutant Mycobacterium smegmatis porin A (MspA) protein nanopore, which is engineered with a histidine at the site 91. MspA nanopore is a channel-forming protein that allows small hydrophilic nutrients to enter the bacterium. Its crystal structure has been characterized using X-ray diffraction (Science, 2004, 303, 1189-1192). The engineered MspA nanopore ( Proc. Natl. Acad. Sci. , 2008, 105, 20647-20652) is widely applied for DNA sequencing (Nat. Biotechnol., 2012, 30, 349-353) because its geometry (1 nm-long, 1.2 nm-wide constriction) provides high spatial resolution. Recently, MspA is also verified to be an excellent nanopore for single-molecule reaction (Nat. Commun, 2019, 10, 5668). In our work, the MspA-H nanopore is designed for coordination study at the single-molecule level. The most sensitive site 91 (the narrowest restriction-1.2 nm wide) of MspA is mutated to the histidine which acts as the coordination site. The coordination reaction between the histidine residue and single monatomic metal ions is systematically studied. The coordination principle in the confined space is unraveled (Chem. Sci., 2020, DOI: 10.1039/C9SC05260G).
Publication	Sha W, Jiao C, Jia W et al. Single molecule observation of hard–soft-acid–base (HSAB) interaction in engineered Mycobacterium smegmatis porin A (MspA) nanopores. Chemical Science, 2020, DOI: 10.1039/C9SC05260G.

Plasmid Copy	High Copy
Bacterial Resistance	Kanamycin
Growth Strain	DH5-Alpha
Growth Temperature	37°C
Plasmid Size (bp)	5814

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