ClyA RR plasmid from Shuo Huang

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Name Cat. No. Availability Price
ClyA RR MC_0068760 4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
$65 Edit in GenSmart Design
This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
  • General
  • Specification
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  • Keywords Other
    Vector Backbone pET-30a(+)
    Source Member Deposit
    Depositor Shuo Huang
    Organization Nanjing University
    Description The plasmid ClyA-RR is designed for the preparation of a mutant Cytolysin A (ClyA). ClyA is a pore-forming toxin which was found in various E. coli strains. Its structure has been characterized using X-ray crystallography (PDB: 2WCD).With a unique wide aperture, ClyA along with its mutants have been used as nanopore sensors for the detection of macromolecules. The ClyA-RR mutant is engineered for more efficient DNA sensing (Franceschini L, Brouns T, Willems K. et al. DNA Translocation through Nanopores at Physiological Ionic Strengths Requires Precise Nanoscale Engineering. ACS Nano 2016, 10(9): 8394-8402). It is also a highly compatible sensor for the imaging based DiffusiOptoPhysiology platform (Wang Y, Wang Y, Du X, et al. Electrode-free nanopore sensing by DiffusiOptoPhysiology[J]. Science Advances, 2019, 5(9): eaar3309.)
    Publication Wang Y, Wang Y, Du X, et al. Electrode-free nanopore sensing by DiffusiOptoPhysiology[J]. Science Advances, 2019, 5(9): eaar3309
    Plasmid Copy Low Copy
    Bacterial Resistance Kanamycin
    Growth Strain TOP10
    Growth Temperature 37°C
    Plasmid Size (bp) 6183
    Gene/Insert Name (ClyA RR)_PET-30a(+)
    Gene/Insert Sequence

    CATATGACAGGAATATTTGCAGAACAAACTGTAGAAGTAGTTAAATCAGCTATTGAGACCGCGGATGGTGCGCTGGACCTGTATAACAAGTACCTGGACCAGGTGATCCCGTGGAAGACCTTCGATGAAACCATTAAGGAACTGAGCCGTTTTAAACAGGAGTATAGCCAAGAAGCGAGCGTGCTGGTTGGTCGTATCAAGGTGCTGCTGATGGACAGCCAGGATAAATACTTCGAGGCGACCCAAACCGTTTATGAATGGGCGGGTGTGGTTACCCAGCTGCTGAGCGCGTACATTCAACTGTTTGACGGCTATAACGAGAAGAAAGCGCGTGCGCAGAAAGATATCCTGATTCGTATCCTGGACGATGGTGTTAAGAAACTGAACGAAGCGCAGAAGAGCCTGCTGACCAGCAGCCAAAGCTTCAACAACGCGAGCGGCAAACTGCTGGCGCTGGACAGCCAACTGACCAACGATTTTAGCGAGAAGAGCAGCTACTATCAGAGCCAAGTGGACCGTATCCGTAAAGAAGCGTACGCGGGTGCGGCGGCGGGTATTGTTGCGGGTCCGTTCGGCCTGATCATTAGCTATAGCATCGCGGCGGGTGTGGTTGAGGGCAAGCTGATTCCGGAACTGAACAACCGTCTGAAAACCGTGCAGAACTTCTTTACCAGCCTGAGCGCGACCGTTAAGCAAGCGAACAAAGACATCGATGCGGCGAAGCTGAAACTGGCGACCGAGATTGCGGCGATCGGCGAAATTAAGACCGAAACCGAAACCACCCGTTTTTACGTGGATTATGACGATCTGATGCTGAGCCTGCTGAAGGGTGCGGCGAAGAAAATGATCAACACCAGCAACGAGTACCAGCAACGTCACGGCCGTAAGACCCTGTTTGAGGTTCCGGACGTGGGTAGCAGCTATCATCATCATCATCATCATTAATGACTCGAG

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