MspA-M plasmid from Shuo Huang

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Name Cat. No. Availability Price
MspA-M MC_0010018 4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
$65 Edit in GenSmart Design
This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
  • General
  • Specification
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  • Keywords Expression
    Vector Backbone pET-30a
    Source Member Deposit
    Depositor Shuo Huang
    Organization Nanjing University
    Description The plasmid MspA-M is designed for the preparation of a mutant Mycobacterium smegmatis porin A (MspA) nanopore, which is engineered with a methionine in the site of 91. Six extra histidine tag are designed for the followup purification using nickel affinity chromatography. MspA is a funnel-shaped, octameric channel protein which measures ~1.2 nm in diameter at its narrowest restriction, and its structure has been characterized using X-ray crystallography (PDB ID: 1uun). With a single, geometrically sharp restriction, MspA has an excellent performance in the DNA sequencing (Manrao E A, Derrington I M, Laszlo A H, et al. Nature biotechnology, 2012, 30(4): 349.; Laszlo A H, Derrington I M, Ross B C, et al. Nature biotechnology, 2014, 32(8): 829.). Rencently, the mutant of MspA (MspA-M) has been reported as a single-molecule nanoreactor for the detection of tetrachloroaurate(III) ions. Its sensing performance is superior to α-hemolysin (α-HL), which indicates that MspA is advantageous for single molecule chemistry investigations and has applications as a hybrid biological nanopore with atomic adaptors (Cao J, Jia W, Zhang J, et al. Nature Communications, 2019, 10(1): 1-11.).
    Publication Cao, J., Jia, W., Zhang, J. et al. Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore. Nat Commun 10, 5668 (2019) doi:10.1038/s41467-019-13677-2
    Plasmid Copy High Copy
    Bacterial Resistance Kanamycin
    Growth Strain DH5-Alpha
    Growth Temperature 37°C
    Plasmid Size (bp) 5810
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