α-HL WT D8H6 plasmid from Shuo Huang

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Name Cat. No. Availability Price
α-HL WT D8H6 MC_0068416 4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
$65 Edit in GenSmart Design
This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
  • General
  • Specification
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  • Keywords Expression
    Vector Backbone
    Source Member Deposit
    Depositor Shuo Huang
    Organization Nanjing University
    Description The plasmid α-HL WT D8H6 is designed for the preparation of the heptameric transmembrane protein alpha-hemolysin (α-HL) WT by prokaryotic expression. A H6 tag is designed for the followup purification using nickel affinity chromatography. α-HL is naturally secreted by Staphylococcus aureus. The structure has been characterized using X-ray crystallography (Song L, Hobaugh M R, Shustak C, et al. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science, 1996, 274(5294): 1859-1865.). Since 1996, α-HL WT along with its mutants have been widely investigated as single-molecule nanopore sensors, suitable for sensing a variety of analyte such as ionic species, small molecules, macromolecules and biomacromolecules.
    Publication Wang Y, Yan S, Zhang P, et al. Osmosis-Driven Motion-Type Modulation of Biological Nanopores for Parallel Optical Nucleic Acid Sensing. ACS applied materials & interfaces, 2018, 10(9): 7788-7797
    Plasmid Copy High Copy
    Bacterial Resistance Kanamycin
    Growth Strain BL21(DE3)
    Growth Temperature 37°C
    Plasmid Size (bp) 6165
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