Proteins, unlike DNA which can be relatively easily synthesized, must be produced using complex mixtures derived from cells or using live cells. There are some types of expression systems used for protein production and purification, including bacterial, insect, mammalian, yeast expression systems, etc.
Each system has advantages and disadvantages, and choosing the right system for the specific application is important for successful recombinant protein expression. These expression systems are summarized below.
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