Phosphodiesterase Screening Services: Accelerate Your Drug Discovery Process

Phosphodiesterases (PDEs) play a pivotal role in cellular signaling by hydrolyzing cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP). These enzymes significantly influence various physiological processes including cell proliferation, inflammation, and neuronal plasticity. As a result, they offer exciting opportunities for novel therapeutic interventions.

Understanding PDEs

This enzyme family consists of multiple families and numerous isoforms, each exhibiting unique tissue distributions, regulatory mechanisms, and substrate preferences. The diverse nature of PDEs presents both challenges and opportunities in drug discovery. Targeted inhibition or activation of specific PDE isoforms can lead to beneficial therapeutic outcomes while limiting off-target effects.

Our Screening Approach

Creative Bioarray employs high-throughput Fluorescence Polarization (FP) technology in our PDE screening services. This advanced method allows quick and accurate assessment of a compound’s inhibitory activity on specific PDE subtypes. The technique is based on the hydrolysis of fluorescently labeled substrates by the target PDE.

In a typical assay, when the substrate remains intact, it binds to specific antibodies, resulting in high fluorescence polarization values. Upon addition of the PDE, the substrate is hydrolyzed, leading to lower polarization values. The introduction of test compounds that inhibit the PDE will maintain higher polarization levels, indicating their effectiveness.

Detection Targets

Our service covers a comprehensive range of PDE subtypes essential for drug development. Key detection targets include:

• PDE1A
• PDE3B
• PDE4B2
• PDE5A1
• PDE9A2
• PDE1B
• PDE4A10
• PDE4C1
• PDE6C
• PDE10A1
• PDE2A1
• PDE4D2
• PDE3A
• PDE4B1
• PDE8A

This coverage ensures that clients can effectively identify and optimize drug candidates against a variety of targets.

Service Features

We provide a suite of advantages designed to enhance the drug discovery process:

• High-Throughput Efficiency: Our technology enables rapid screening of vast compound libraries, significantly shortening discovery timelines.
• Data Precision and Reliability: Rigorous validation processes ensure accuracy and reproducibility of results.
• Expert Technical Support: Our team comprises seasoned professionals in pharmacology and enzymology, offering tailored experimental design and data analysis.
• Flexible Custom Solutions: We understand the complexities of drug discovery and can adapt services to meet specific project requirements.

Final Thoughts

Engaging in phosphodiesterase screening services can lead to groundbreaking advancements in therapeutic strategies, particularly in fields such as oncology, cardiology, and neurology. Reach out to our customer service representatives available around the clock, ready to assist with inquiries and provide tailored support.