iPSC Characterization

iPSC generation has become increasingly easier because of advanced technology, but the characteristics of various iPSCs is depending on the source, derivation, passage and culture conditions. In order to evaluate the pluripotency, safety and confirm the identity of PSCs, it is essential to perform a set of characterization analysis. Our scientists with extensive experience at Creative Bioarray provide comprehensive iPSC characterization service for customers all over the world.

Creative Bioarray Offers iPSC Characterization Service as Follows (including but not limited to):

1. Pluripotency and lineage-specific markers staining:

• Alkaline phosphatase live staining
• Immunocytochemistry for key pluripotency markers
• qPCR and RNA-seq
• TaqMan hiPSC scorecard assay

2. Karyotyping:

• Comparative genomic hybridization analysis
• G-banding
• Multicolor FISH (M-FISH) analysis
• Chromosomal microarray analysis
• q-PCR analysis

3. Differentiation potential analysis:

• Potential of directed differentiation into specific lineages
• Embryoid body (EB) formation
• Teratoma formation assay

4. Microbiological contamination testing:

• Mycoplasma testing
• Pathogen testing

5. Genome sequencing analysis:

• Whole genome sequencing
• Exome gene sequencing
• cDNA expression analysis

6. HLA typing for potential transplantation studies
7. DNA Fingerprinting (Short tandem repeats (STR) profiling)