Digital ISH Image Quantification and Statistical Analysis

In situ hybridization (ISH, CISH, FISH) is a popular approach for the evaluation of changes in biomarker expression or a particular drug response effect in tissues, especially in the case of novel biomarkers where robust, well validated antibodies are not commercially available. And investigating RNA at a single cell level can give important information about a cell’s dynamic gene expression. When analyzed in situ and in the context of the whole tissue, researchers can identify aberrant gene expression associated with disease states, such as cancer and neurological disease.

DNA FISH analysis is also available in Creative Bioarray include the FISH Amplification & Deletion Analysis module, used to quantify up to two fluorescently labeled DNA probes to measure amplification or deletion, and the FISH Break apart & Fusion Analysis module, used to measure gene rearrangements detected using break-apart fusion probes.