CRISPR Ribonucleoprotein (RNP) User Manual

A guide on how to use CRISPR RNP for targeted genome editing.

What is included?

1. How to prepare RNA oligos
2. How to prepare RNP complex
3. How to test RNP efficacy in cells, through either cationic lipid-mediated transfection or electroporation
4. How to test RNP efficacy in embryos
5. How to test RNP in vitro cutting efficiency

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