Blood Plasma Partitioning Assay

Creative Bioarray provides Plasma Protein Binding Assay to help customers accurately detect the concentration of the drug in whole blood compared to plasma, predicting the binding of the compound to red blood cells.

Why do we need Blood Plasma Partitioning Assay?

• Information on the partitioning of a pharmaceutically important molecule in red blood cells (RBC) is critical for understanding the compound's pharmacokinetics and pharmacodynamics and is sometimes critical in selecting a therapeutic candidate without missing out.
• Blood determines the drug concentration ratio in whole blood to plasma, revealing the binding of the drug to red blood cells.
• The pharmacokinetic parameters computed from plasma data may be misleading if there is a discrepancy in drug concentration between plasma and red blood cells due to different binding to specific components in the blood.
• When the blood to plasma ratio is greater than 1 (usually the result of the distribution of the drug into the red blood cells), the plasma clearance rate significantly overestimates the blood clearance rate and may exceed the hepatic blood flow. The ratio of blood to plasma can also be used to understand potential blood toxicity.

Brief Protocol

• The test compound is added to fresh heparinized whole blood, reference red blood cells, and reference plasma.
• After the incubation period, the whole blood is centrifuged.
• Two parts of whole blood (plasma and red blood cells) are analyzed by LC-MS/MS together with the reference sample.
• The following equation calculates the ratio of blood to plasma.