Interested in a method or technology as an alternative to drug selection or dilution cloning to select positive clones expressing a gene such as FLP-FRT recombination or CRISPR ideally with a platform with bioluminescence or non-fluorescents alternative. Are any cell sorting machines useful for non-fluorescents capabilities?
Any help or advice would be much appreciated.
Thanks
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Very interesting question! Maybe you could try the microfluidic chip, flow cytometry or a combination method with antibody and magnetic beads.