To design a membrane protein with N terminal tagged Flag, how to put the signal peptide?
Mark the position of cleavage of your signal peptide. Put the tag just after the cleavage site. So, after the cleavage, the tag will be there! I hope this helps.
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Mark the position of cleavage of your signal peptide. Put the tag just after the cleavage site. So, after the cleavage, the tag will be there! I hope this helps.