pLentiCRISPR v2 SARS is a gRNA and WT SpCas9 co-expression plasmid that targets the SARS gene of human genome.

gRNA and WT SpCas9 co-expression plasmid: pLentiCRISPR v2 SARS

Reference Sequence View the backbone

pLentiCRISPR v2 SARS

Cat. No.:

MC_0064451

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$99

General

Specification

Comments (0)

Keywords	CRISPR
Gene Targeted	SARS
Gene Target Sequence	TGTACCCATCAGTAACGATG
Species	H. sapiens (human)
Description	The following gRNA sequence is designed by Feng Zhang’s laboratory at the Broad institute to uniquely target the indicated gene within the human genome. The gRNA sequence is for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. The sgRNA sequence was validated by Feng Zhang’s research.
Vector Backbone	pLentiCRISPR v2
Selectable Marker	Puromycin
Reference	Sanjana N.E., Shalem O., Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods (2014) 11(8): 783-4.

Plasmid Copy	High Copy
Bacterial Resistance	Ampicillin
Growth Strain	Stbl3
Growth Temperature	37°C
Plasmid Size (bp)	13013
Gene/Insert Name	SARS
Gene/Insert Sequence	TGTACCCATCAGTAACGATG

Customers who viewed this item also viewed:

OMu00440

Mus musculus defensin beta 43 (Defb43), mRNA.

pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)

Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).

2019-nCov_pcDNA3.1(+)-P2A-eGFP

The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.

SARS_pcDNA3.1(+)-P2A-eGFP

The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.

2019-nCov-Linker_pcDNA3.1(+)-C-eGFP

The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.

SARS-Linker_pcDNA3.1(+)-C-eGFP

The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.