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pLentiCRISPR v2 SARS
The following gRNA sequence is designed by Feng Zhang’s laboratory at the Broad institute to uniquely target the indicated gene within the human genome. The gRNA sequence is for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. The sgRNA sequence was validated by Feng Zhang’s research.
2019-nCov_pcDNA3.1(+)-P2A-eGFP
The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
SARS_pcDNA3.1(+)-P2A-eGFP
The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
2019-nCov-Linker_pcDNA3.1(+)-C-eGFP
The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.
SARS-Linker_pcDNA3.1(+)-C-eGFP
The plasmid is used for SARS surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through linker, which can be used for flow cytometry antibody screening.