pCAS-ZE3 plasmid from Sheng Yang
| Keywords | CRISPR |
| Vector Backbone | pCAS-RK2T |
| Source | Member Deposit |
| Depositor | Sheng Yang |
| Organization | Center for Excellence in Molecular Plant Sciences (CEMPS), CAS |
| Description | E. coli-Pseudomonas putida KT2440 shuttle plasmid containing dCas9 and sgRNA targeting the J5 constitutive promoter |
| Publication | J. Sun, Q. Wang, Y. Jiang, Z. Wen, L. Yang, J. Wu, and S. Yang, \\\'Genome Editing and Transcriptional Repression in Pseudomonas Putida Kt2440 Via the Type Ii Crispr System\\\', Microb Cell Fact, 17 (2018), 41. |
| Plasmid Copy | Low Copy |
| Bacterial Resistance | Tetracycline |
| Growth Strain | DH5-Alpha |
| Growth Temperature | 37°C |
| Plasmid Size (bp) | 13881 |
| Gene/Insert Name | dCas9 |