This phagemid can be used for cloning to construct the phage display library. Moreover, it can be used as expression vector for recombinant antibody production when transform into non-Suppressor host cell (E.coli HB2151)
Publication
Pansri, P., Jaruseranee, N., Rangnoi, K., Kristensen, P. and Yamabhai, M., 2009. A compact phage display human scFv library for selection of antibodies to a wide variety of antigens. BMC biotechnology, 9(1), p.6.
Plasmid Copy
High Copy
Bacterial Resistance
Ampicillin
Growth Strain
TG1/DH5alfa F'
Growth Temperature
37°C
Plasmid Size (bp)
4540
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This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR
The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.