pNICKclos1.0 plasmid from Sheng Yang
| Keywords | CRISPR |
| Vector Backbone | pIMP1-ptb |
| Source | Member Deposit |
| Depositor | Sheng Yang |
| Organization | Center for Excellence in Molecular Plant Sciences (CEMPS), CAS |
| Description | Composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging |
| Publication | Q. Li, J. Chen, N. P. Minton, Y. Zhang, Z. Wen, J. Liu, H. Yang, Z. Zeng, X. Ren, J. Yang, Y. Gu, W. Jiang, Y. Jiang, and S. Yang, \\\'Crispr-Based Genome Editing and Expression Control Systems in Clostridium Acetobutylicum and Clostridium Beijerinckii\\\', Biotechnol J, 11 (2016), 961-72. |
| Plasmid Copy | Low Copy |
| Bacterial Resistance | Ampicillin |
| Growth Strain | DH5-Alpha |
| Growth Temperature | 37°C |
| Plasmid Size (bp) | 11081 |
| Gene/Insert Name | Cas9 nickase |