Dr. Bankala Krishnarjuna’s team recently published an article titled “Detergent-free extraction, reconstitution and characterization of membrane-anchored cytochrome-b5 in native lipids” in Chemical Communications, introducing an improved method for membrane protein extraction without destabilizing protein structures (Chemical Communications, 2020). In this study, the researchers demonstrated the feasibility of a direct extraction of a B16 kDa cytochrome-b5 from E. coli using a synthetic styrene-co-maleic acid-ethanolamine (SMA-EA) polymer and reconstituting the cytochrome-b5 protein in polymer nanodiscs along with native lipids from E. coli.
With the help of Dr. Bankala Krishnarjuna, MolecularCloud also initiated a reward-winning campaign for this specific topic "Membrane Protein Extraction", which attracted 63 participants and have been discussed by 24 users, 2 among which with the first perfect quiz score or the best comment have won the reward.
Winners of the Membrane Protein Extraction Campaign
Congratulations to our winners! Each of the winners can choose one of the following gifts (a canvas bag, hoodie or $20 Amazon gift card):