what is yeast two-hybrid

The yeast two-hybrid system, also known as interaction trap, is an effective method for the study of eukaryotic living cells.Galactose metabolism in yeast genes are needed for the two regulatory proteins and GAl4 GAL80 regulation, when existing galactose, GAl4 protein and UAS GAl4 effect in the upstream of the gene and the original combination, activation of transcription, there is no galactose, GAL80 and GAl4 hinder the transcription activation, complete yeast transcription factor GAl4 in two domain structure, a dna-binding domain, a transcription activation domain.Yeast two-hybrid is composed of three parts: one is the protein expression vector fused with BD, the expressed protein is the bait protein, and the other is the protein expression vector fused with AD, the target protein, and the strain with one or more reporter genes.Known as bait protein X protein, unknown Y protein as the target protein, and respectively and BD, AD reconnection and report gene activation sequence upstream UAS is united in wedlock, if XY can be formed between the protein complexes, and make the AD, BD close to each other, transcription, make the UAS downstream promoter regulation report gene express it, on the contrary, if unable to interaction between the bait protein with its target protein, the report can't be expressed genes.