MolecularCloud May Newsletter: SpCas9-NG self-targets the sgRNA sequence in plant genome editing

SpCas9-NG self-targets the sgRNA sequence in plant genome editing


Dr. Pengcheng Wei’s team published the paper “SpCas9-NG self-targets the sgRNA sequence in plant genome editing ” at Nature Plants in 24 February 2020. The plasmid pHUC411-gcccc and pHUC411NG-gcccc  of this article have been deposited on MolecularCloud.

 


Streptococcus pyogenes Cas9 (SpCas9)-NG recognizes NGN protospacer adjacent motifs and expands the scope of genome-editing tools. 


In this study, Dr. Pengcheng Wei found that SpCas9-NG not only targeted the genome but also efficiently self-targeted the single-guide RNA sequence in transfer DNA in transgenic plants, potentially increasing off-target risk by generating new single-guide RNAs. They further showed that the self-target effect of SpCas9-NG could be greatly alleviated by using a modified single-guide RNA scaffold starting with a GCCCC sequence.


Plasmids from this article in MolecularCloud

Cat. No.

Plasmid Name

Description

MC_0101108

pHUC411-gcccc

SpCas9 binary vector with a modified sgRNA scaffold starting with a GCCCC sequence

MC_0101109

pHUC411NG-gcccc

SpCas9-NG binary vector with a modified sgRNA scaffold starting with a GCCCC sequence


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