Molecular Characterization Testing

Creative Biogene has gathered a number of senior experts to provide the molecular characterization testing service to clients with stable quality and reasonable price. Creative Biogene is based on the frontiers of transgenic plant service with complete transformation system and years of service experience. And we have mature screening and testing technology for transgenic plants.

The molecular characterization testing of transgenic plants is an important step to determine whether the target gene has been transformed into the plants successfully. The molecular characterization testing of genetically modified organisms is of great importance. It is commonly applied to the safety assessment of genetically modified organism, laying the foundation for the subsequent monitor of genetically modified crops.

DNA Level

• PCR

PCR screening is one of the most widely used methods because of the high efficiency, simple operation and low cost. PCR is an enzymatic reaction using DNA polymerase to amplify the target fragment. When it is used for analyzing transgenic plants, a band in specific size will be in the gel if the target gene is transformed into the plants successfully.

• Southern Blotting

Southern Blotting is an assay utilizing labeled probes to specifically hybridize with the target DNA, which can be used for analyzing the integration of foreign genes on plant chromosomes and the stability of foreign genes in transgenic offspring. The southern blotting is used in molecular characterization of transgenes to determine the existence and copy numbers of the introduced gene into the GMO genome. It has been extensively used in the screening of transgenic plants of various crops such as rice, wheat, corn, soybean, rapeseed, peach with high accuracy and specificity.