Explore Creative Biogene’s Recombinase AAV Particles for Advanced Genome Engineering

Recombinase AAV Particles represent a significant advancement in genome engineering and therapeutic applications. Developed by Creative Biogene, these particles incorporate essential genome engineering proteins, including Cas9, Cpf1, Cre, and FLPo, facilitating precise manipulation of cell genomes.

Product Overview

Creative Biogene offers a comprehensive selection of recombinase AAV particles designed for targeted DNA cleavage and recombination events. The quality and efficacy of these particles have been validated through extensive testing in mammalian cells, ensuring reliable results for researchers and practitioners alike.

Key Advantages:

• Broad Range of Applications: Suitable for gene function studies, gene therapy, and the creation of animal models.
• Robust Viral Production: Utilizes triple-plasmid transfection methods for high-yield viral production.
• Custom Solutions: Options for tailored recombinant AAV services to fit specific research needs.
• Tissue-Specific or Inducible Promoters: Achieve tight regulation of recombinase expression.

Benefits of Recombinase AAV Particles

• Diverse Capabilities: Capable of executing precise genome editing via nucleases and recombinases.
• Effective Delivery: High delivery efficiency ensures that therapeutic agents reach target cells effectively.

Applications

Recombinase AAV particles are instrumental in various applications:

• Genome Editing: Use of Cas9 and Cpf1 for site-specific DNA modifications.
• Gene Function Research: Analyze gene behavior in specific cell types post-gene knockout.
• Animal Models: Rapid generation of genetically modified organisms for experimental purposes.
• Gene Therapy: Direct correction of genetic mutations responsible for diseases.

Product List

Explore a variety of AAV options including:

• Cre Adeno-associated virus (AAV Serotype 5): Product Link
• Cre Adeno-associated virus (AAV Serotype 1): Product Link
• AAV9-CMV-Cas9: Product Link

Case Studies

1. Social Behavior Alterations: Research using AAV-shSRF demonstrated impacts on dendritic spine morphology in neurons, linking serum response factor to structural changes.

2. Astrocyte Apoptosis in the Hippocampus: A study utilized AAV-Cre to induce astrocyte apoptosis, providing insight into recovery mechanisms for reactive gliosis.

3. Nutrient Sensing in Liver Metabolism: The role of SUMOylation in regulating fasting metabolism was explored using AAV-Cre in mouse models, revealing crucial insights into liver function.

Frequently Asked Questions

Q: What are Recombinase AAV particles?
A: These particles deliver site-specific recombinases to catalyze genetic modifications, allowing for controlled gene editing.

Q: How are these particles administered?
A: Through a multi-step process of binding, endocytosis, and nuclear translocation, the AAV capsid delivers its genetic payload efficiently.

Q: What are the advantages of using rAAV vectors?
A: They offer low immunogenicity, the ability to transduce both dividing and non-dividing cells, and long-term expression of transgenes.