Q: What vector can be used to clone large DNA fragment?
Bacterial artificial chromosomes (BACs) are simple plasmid which is designed to clone very large DNA fragments ranging in size from 75 to 300 kb.
A very large DNA fragments whose sizes ranging from 100 kb to 3000 kb can be cloned using Yeast Artificial Chromosomes.
Human artificial chromosomes (HACs) or mammalian artificial chromosomes (MACs) are still under development.HACs are microchromosomes that can act as a new chromosome in a population of human cells.HACs range in size from 6 to 10 Mb that carry new genes introduced by human researchers.