Escherichia coli Genome Editing Service

Creative Biogene is a leading biotechnology company offering the best E.coli genome editing services. With years of experience and expertise in microbial genome editing, our talented scientists will work closely with you to provide any help in E.coli genome editing services.

Escherichia coli is one of the most extensively studied bacteria that becomes an instrumental model system for the understanding of a plethora of gene functions and regulations in both prokaryotes and eukaryotes. In addition, it's a very versatile host for the production of heterologous proteins and their mass-production in industrial fermentation systems. E.coli also plays an invaluable role in modern biological engineering and industrial microbiology. More applications are expected to be developed due to the modern molecular technique of the genome editing.

Our E.coli genome editing  ervices are based on CRISPR/Cas9 technology and homologous recombination technique. The state-of-art E.coli editing system helps you successfully achieve gene knockout, gene insertion and point mutation for either research or industrial purposes.

E.coli Genome Editing Based on Homologous Recombination

Red/ET Recombination permits the engineering of DNA in E.coli using homologous recombination mediated by phage protein pairs, either RecE/RecT or Reda/Redb. The central step in Red/ET recombination is the crossover step between a targeting construct containing homology arms and the target which can be a gene locus on the E.coli chromosome by designing a homologous fusion fragment of the target gene, it is cloned into a suicide vector, and the suicide vector is transformed into the target bacterium. An insertion mutant is selected by antibiotic screening. Under the second round of reverse selection pressure, only the mutation that contain second homologous recombination and the loss of the suicide plasmid can survive. By PCR screening and sequencing, we can obtain the mutant of the strain.