Advanced circRNA Luciferase Reporter Assay Service for Target Validation and Binding Site Discovery

IntegrateRNA offers a specialized circRNA luciferase reporter assay service aimed at validating circRNA targets and discovering detailed binding sites crucial for your molecular biology research. Utilizing cutting-edge molecular biology platforms and a team of skilled scientists, we provide innovative solutions with guaranteed quality and competitive pricing. Our commitment is to advance your basic research and streamline drug discovery efforts.

Understanding Circular RNAs

Circular RNAs (circRNAs) are unique molecular entities, featuring covalently closed and single-stranded structures derived from precursor mRNA back splicing. Recent advancements in high-throughput RNA sequencing technologies and bioinformatics have allowed for the identification of thousands of circRNAs across various species. Emerging studies emphasize the roles of circRNAs as regulators of transcription and splicing, particularly their interactions with microRNAs (miRNAs), acting as sponges to modulate miRNA activity.

The Role of Luciferase Reporter Assays

The luciferase reporter assay stands as a pivotal tool in gene expression studies at the transcriptional level. This technique allows researchers to explore the regulatory effects of miRNAs on specific circRNAs. By constructing a luciferase vector—usually utilizing a vector like psiCHECK2—researchers can insert the circRNA sequence into the 3’ UTR of a promoter-driven luciferase reporter gene.

How It Works

Cells are co-transfected with the luciferase reporter vector and the miRNA mimics. The resultant luciferase activity is then compared against negative controls to evaluate whether the circRNA acts as a sponge for the miRNA. If the interaction is confirmed, a decrease in luciferase activity indicates the binding of the miRNA to the circRNA. Conversely, if an engineered mutant sequence of circRNA is used, any change in luciferase activity can elucidate specific binding sites involved in the interaction.

Benefits of the Assay

• Instantaneous Measurements: The luciferase activity can be measured almost immediately.
• High Sensitivity: It provides exceptional sensitivity for detecting interactions.
• Wide Dynamic Range: The assay accommodates a broad range of expression levels.
• Versatility: Compatible with various cell lines for diverse experimental setups.
• One-Stop Solution: All necessary components are provided, simplifying your research process.
• Quick Turnaround: Rapid results to expedite your research timeline.

Assay Workflow

Utilizing our dual-luciferase reporter assay specifically designed for circRNA research, we facilitate rigorous investigation into targeting miRNAs and their binding sites. By supplying the sequence information of the circRNA and/or the associated miRNAs, you can expect professional, reliable, and timely experimental outcome