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CRISPR 10 ternary vector system 7 Genome Editing 5
Introduction of the prime editing vectors developed in Chen Lab Recently, a novel, universal, and precise genome-editing technology named prime editing was developed (Anzalone et al. Nature. 2019, 576: 149–57). We generated a set of pGreen3 binary vectors for prime editing in ...Learn More
Agrobacterium-mediated delivery of CRISPR/Cas reagents for genome editing in plants enters an era of ternary vector systems Lack of appropriate methods for delivery of genome-editing reagents is a major barrier to CRISPR/Cas-mediated genome editing in plants. ...Learn More
Our current research focuses on the plant gene editing with the ternary vector system. Recently, we developed a novel ternary vector system to integrate the MR (Morphogenic Regulator) and CRISPR/Cas modules. This ternary vector system is composed of new pGreen-like binary ...Learn More
Qiang Zhang, Yu Zhang, Min-Hui Lu, Yi-Ping Chai, Yuan-Yuan Jiang, Yun Zhou, Xue-Chen Wang, Qi-Jun Chen. (2019). A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiology. DOI: 10.1104/pp.19.00767.
Q. Zhang, H. L. Xing, Z. P. Wang, H. Y. Zhang, F. Yang, X. C. Wang, and Q. J. Chen, 'Potential High-Frequency Off-Target Mutagenesis Induced by Crispr/Cas9 in Arabidopsis and Its Prevention', Plant Mol Biol, 96 (2018), 445-56.
Chen, Y., Wang, Z., Ni, H., Xu, Y., Chen, Q., and Jiang, L. (2017). CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci.
Zhang, H.Y., Wang, X.H., Dong, L., Wang, Z.P., Liu, B., Lv, J., Xing, H.L., Han, C.Y., Wang, X.C., and Chen, Q.J. (2017). MISSA 2.0: an updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems. Sci Rep 7, 41993.
Wang, Z.P., Xing, H.L., Dong, L., Zhang, H.Y., Han, C.Y., Wang, X.C., and Chen, Q.J. (2015). Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol 16, 144.
Xing, H.L., Dong, L., Wang, Z.P., Zhang, H.Y., Han, C.Y., Liu, B., Wang, X.C., and Chen, Q.J. (2014). A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol 14, 327.
Chen, Q.J., Xie, M., Ma, X.X., Dong, L., Chen, J., and Wang, X.C. (2010). MISSA is a highly efficient in vivo DNA assembly method for plant multiple-gene transformation. Plant Physiol 153, 41-51.
An, R., Chen, Q.J., Chai, M.F., Lu, P.L., Su, Z., Qin, Z.X., Chen, J., and Wang, X.C. (2007). AtNHX8, a member of the monovalent cation: proton antiporter-1 family in Arabidopsis thaliana, encodes a putative Li/H antiporter. Plant J 49, 718-728.
Chen, Q.J., Zhou, H.M., Chen, J., and Wang, X.C. (2006). A Gateway-based platform for multigene plant transformation. Plant Mol Biol 62, 927-936.
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