Qijun Chen

Dr. Qijun Chen obtained the doctoral degree at the China Agricultural University in 2002. Since September 2004, he has been working in the College of Biological Sciences, China Agricultural University. Dr. Chen's current research focuses on the plant gene editing with the ternary vector system. Recently, his lab developed a novel ternary vector system to integrate the MR(Morphogenic Regulator) and CRISPR/Cas modules. This ternary vector system is composed of new pGreen-like binary vectors including pGreen3 and a pVS1-based virulence helper plasmid, which also functions as a replication helper for the pGreen3 vectors in Agrobacterium tumefaciens

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College of Biological Sciences, China Agricultural University

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CRISPR 10 ternary vector system 7 Genome Editing 5

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Agrobacterium-mediated delivery of CRISPR/Cas reagents for genome editing in plants enters an era of ternary vector systems Lack of appropriate methods for delivery of genome-editing reagents is a major barrier to CRISPR/Cas-mediated genome editing in plants. ...Learn More


Our current research focuses on the plant gene editing with the ternary vector system. Recently, we developed a novel ternary vector system to integrate the MR (Morphogenic Regulator) and CRISPR/Cas modules. This ternary vector system is composed of new pGreen-like binary ...Learn More


  1. Qiang Zhang, Yu Zhang, Min-Hui Lu, Yi-Ping Chai, Yuan-Yuan Jiang, Yun Zhou, Xue-Chen Wang, Qi-Jun Chen. (2019). A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize. Plant Physiology. DOI: 10.1104/pp.19.00767.

  2. Q. Zhang, H. L. Xing, Z. P. Wang, H. Y. Zhang, F. Yang, X. C. Wang, and Q. J. Chen, 'Potential High-Frequency Off-Target Mutagenesis Induced by Crispr/Cas9 in Arabidopsis and Its Prevention', Plant Mol Biol, 96 (2018), 445-56.

  3. Chen, Y., Wang, Z., Ni, H., Xu, Y., Chen, Q., and Jiang, L. (2017). CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci.

  4. Zhang, H.Y., Wang, X.H., Dong, L., Wang, Z.P., Liu, B., Lv, J., Xing, H.L., Han, C.Y., Wang, X.C., and Chen, Q.J. (2017). MISSA 2.0: an updated synthetic biology toolbox for assembly of orthogonal CRISPR/Cas systems. Sci Rep 7, 41993.

  5. Wang, Z.P., Xing, H.L., Dong, L., Zhang, H.Y., Han, C.Y., Wang, X.C., and Chen, Q.J. (2015). Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol 16, 144.

  6. Xing, H.L., Dong, L., Wang, Z.P., Zhang, H.Y., Han, C.Y., Liu, B., Wang, X.C., and Chen, Q.J. (2014). A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol 14, 327.

  7. Chen, Q.J., Xie, M., Ma, X.X., Dong, L., Chen, J., and Wang, X.C. (2010). MISSA is a highly efficient in vivo DNA assembly method for plant multiple-gene transformation. Plant Physiol 153, 41-51.

  8. An, R., Chen, Q.J., Chai, M.F., Lu, P.L., Su, Z., Qin, Z.X., Chen, J., and Wang, X.C. (2007). AtNHX8, a member of the monovalent cation: proton antiporter-1 family in Arabidopsis thaliana, encodes a putative Li/H antiporter. Plant J 49, 718-728.

  9. Chen, Q.J., Zhou, H.M., Chen, J., and Wang, X.C. (2006). A Gateway-based platform for multigene plant transformation. Plant Mol Biol 62, 927-936.


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