There are two methods to purify polyclonal antiserum or monoclonal ascites tissue culture supernatant. Protein A/G (Protein A or protein G) purification is mainly to utilize the high affinity of Staphylococcus aureus protein A or Staphylococcus protein G to the immunoglobulin Fc ...Learn More
Proteins, unlike DNA which can be relatively easily synthesized, must be produced using complex mixtures derived from cells or using live cells. There are some types of expression systems used for protein production and purification, including bacterial, insect, mammalian, yeast ...Learn More
Today, I saw an interesting question to share with you here. What if the protein can't be expressed and purified in E. coli system? There are a few things will do to solve the problem: (1) codon optimization is recommended if it has not been; (2) remove the transmembrane ...Learn More
If you missed A Guide on RNP System for CRISPR Editing(1), we will attach it here. You can find the information about how to prepare RNA oligos and RNP complex. Today, this issue covers topics on：l How to test RNP efficacy in cells, through either cationic lipid-mediated ...Learn More
Using CRISPR ribonucleoprotein (RNP) system for genome editing has many advantages over the traditional CRISPR plasmid method, including improved transfection efficiency in hard-to-transfect cells and reduced off-target effects. Firstly, you need to know How to prepare RNA oligos ...Learn More
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