pUC-45-POTt plasmid from Tian-Qiong Shi

Sequence by GenScript Sequence by Depositor

pUC-45-POTt

Cat. No.:

MC_0101321

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$65

Edit in GenSmart Design

General

Specification

Comments (0)

Keywords	Cloning
Vector Backbone	pUC57-T7-FCC1
Source	He Huang Lab
Depositor	Tian-Qiong Shi
Organization	Nanjing Normal University
Description	bsaI-4-POTt-5-bsaI
Publication	Li YW, Yang CL, Shen Q, Peng QQ, Guo Q, Nie ZK, Sun XM, Shi TQ, Ji XJ, Huang H. YALIcloneNHEJ: An Efficient Modular Cloning Toolkit for NHEJ Integration of Multigene Pathway and Terpenoid Production in Yarrowia lipolytica[J]. Front Bioeng Biotechnol. 2022, 9:817016

Plasmid Copy	High Copy
Bacterial Resistance	Ampicillin
Growth Strain	DH5-Alpha
Growth Temperature	37°C
Plasmid Size (bp)	3700
Gene/Insert Name	45-POTt
Gene/Insert Sequence	GGTCTCCCCACATGTACATACAAGATTATTTATAGAAATGAATCGCGATCGAACAAAGAGTACGAGTGTACGAGTAGGGGATGATGATAAAAGTGGAAGAAGTTCCGCATCTTTGGATTTATCAACGTGTAGGACGATACTTCCTGTAAAAATGCAATGTCTTTACCATAGGTTCTGCTGTAGATACTTGTACAGTTGCAGACCAGTTGGAGTATAGAATGGTACACTTACCAAAAAGTGTTGATGGTTGTAACTACGATACGGGATCCCCGCTGATATGCCTAAGGAACAATCAAAGAGGAAGATATTAATTCAGAATGCTAGTATACAGTTAGGGATGGTGAGTGTTGGGTGATGCAGGGGAGGTTGTCGAGCGGTTCACTTGAGTGCGTCCCGCTGTTTCATCAAGTGCATTTCAAGAATCCCGTTGGCCATGTGCATTTTGACCTTTTGCGGGTCGATTTTGACCGTTGGCGGAAACCGCACCGTTCTCTGGAAGGAGCCGTTGTGTCGTTCTCCCACCATGAGACAGTCGGACAGATGTGCAAAGGGGTTGGTGTAGTGGCCACTGATGGTCAGGCTGTTGGAGTCTGCGTCGTAGGCGGTTTCCAGGTCCGCCGACCGCACCGCCGGCAGACTTGTCACCACAATGTACATGTTTCCCAGGTCGTAGATGTCGACTGGTGGCGCGAATGGGTCAGTATGGGAGTGTGGGAGAGGAGTAGCTGGTGGCGAGTGGGCCAGGTCTGTTTCGGCATAGTCGGGCATTACAAAGGACATGGGGAGGGCGACGGGAGGCTTTTTGAGGAGTTGAAGAAGAGCTGGGGAAGAAATCAAGTGTCAAGCTTGCCTCGAGACC

*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.

Customers who viewed this item also viewed:

pRed_Cas9_recA_∆poxb300

CRISPR

pRed_Cas9_∆poxB300

CRISPR

pCas

Constitutive expression of Cas9 together with inducible expression of lambda RED and sgR

pCAGO

CRISPR/Cas9-assisted gRNA-free one-step genome editing

pUC57-2019-nCoV-PC:RdRP

This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR

pUC57-2019-nCoV-S(Original)

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (original sequence)

pUC57-2019-nCoV-S(Human)

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Human expression system)

pUC57-2019-nCoV-N

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) nucleocapsid phosphoprotein (original sequence)

pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)

Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).

2019-nCov_pcDNA3.1(+)-P2A-eGFP

The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.