pCas

Cat. No.: MC_0000011
   Sequence by GenScript        Sequence by Depositor

Ordering:

Name Cat. No. Availability Price
pCas MC_0000011 4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
$65 Edit in GenSmart Design
This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
  • General
  • Specification
  • Comments (1)
  • Keywords CRISPR
    Vector Backbone pKD46
    Source Member Deposit
    Depositor Sheng Yang
    Organization Shanghai Institutes for Biological Sciences, CAS
    Description Constitutive expression of Cas9 together with inducible expression of lambda RED and sgR
    Publication Y. Jiang, B. Chen, C. Duan, B. Sun, J. Yang, and S. Yang, \'Multigene Editing in the Escherichia Coli Genome Via the Crispr-Cas9 System\', Appl Environ Microbiol, 81 (2015), 2506-14.
    Plasmid Copy Low Copy
    Bacterial Resistance Kanamycin
    Growth Strain DH5-Alpha
    Growth Temperature 30°C
    Plasmid Size (bp) 12542
    Gene/Insert Name cas9
    Customers who viewed this item also viewed:

    pUC57-2019-nCoV-PC:RdRP

    This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR

    pUC57-2019-nCoV-PC:N

    This plasmid contains part of 2019-nCoV(SARS-CoV-2) N gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR

    pUC57-2019-nCoV-PC:E

    This plasmid contains part of 2019-nCoV(SARS-CoV-2) E gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR

    pUC57-2019-nCoV-S(Original)

    This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (original sequence)

    pUC57-2019-nCoV-S(Human)

    This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Human expression system)

    pUC57-2019-nCoV-S(E. coli)

    This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for E. Coli expression system)

    pUC57-2019-nCoV-N

    This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) nucleocapsid phosphoprotein (original sequence)

    pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)

    Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).

    2019-nCov_pcDNA3.1(+)-P2A-eGFP

    The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.
    #[first_name]
    #[create_date]
    #[comment]
    Reply(#[reply_num])
    #[like_num]
  • #[first_name]
    #[create_date]
    #[comment]
    Reply
    #[like_num]