pTet-dCas9-C5-gldA-prpE plasmid from Taek Soon Lee

Sequence by GenScript Sequence by Depositor

pTet-dCas9-C5-gldA-prpE

Cat. No.:

MC_0010062

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$65

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General

Specification

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Keywords	Expression
Vector Backbone
Source	Member Deposit
Depositor	Taek Soon Lee
Organization	The Joint BioEnergy Institute (JBEI) - CA
Description	dCas9 under engineered Ptet promoter, gRNA under PJ23119, targeting gldA, prpE genes
Publication	Tian, T., Kang, J. W., Kang, A., & Lee, T. S. (2019) "Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli"

Plasmid Copy	Low Copy
Bacterial Resistance	Kanamycin
Growth Strain	DH10B
Growth Temperature	37°C
Plasmid Size (bp)	9550

*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.

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