pTet-dCas9-C5-P1 plasmid from Taek Soon Lee
| Keywords | Expression |
| Vector Backbone | |
| Source | Member Deposit |
| Depositor | Taek Soon Lee |
| Organization | The Joint BioEnergy Institute (JBEI) - CA |
| Description | dCas9 under wild type Ptet promoter, gRNA under PJ23119, targeting PJ23100 promoter |
| Publication | Tian, T., Kang, J. W., Kang, A., & Lee, T. S. (2019) "Redirecting Metabolic Flux via Combinatorial Multiplex CRISPRi-Mediated Repression for Isopentenol Production in Escherichia coli" |
| Plasmid Copy | Low Copy |
| Bacterial Resistance | Gentamycin |
| Growth Strain | DH10B |
| Growth Temperature | 37°C |
| Plasmid Size (bp) | 9286 |