pQCascade plasmid from Sheng Yang
| Keywords | CRISPR |
| Vector Backbone | pCDFDuet-1 |
| Source | |
| Depositor | Sheng Yang |
| Organization | Center for Excellence in Molecular Plant Sciences (CEMPS), CAS |
| Description | pCDFDuet-1 harboring TniQ, Cas8, Cas7 and Cas6 from V. cholerae strain HE-45 and inserting BsaI restriction sites flanked by two CRISPR repeats |
| Publication | Zhang, Y., Sun, X., Wang, Q., Xu, J., Dong, F., Yang, S., ... & Zhang, J. (2020). Multicopy chromosomal integration using CRISPR-associated transposases. ACS synthetic biology. |
| Plasmid Copy | High Copy |
| Bacterial Resistance | Streptomycin |
| Growth Strain | DH5-Alpha |
| Growth Temperature | 37°C |
| Plasmid Size (bp) | 8493 |