pLentiCRISPR v2 DNMT1 is a gRNA and WT SpCas9 co-expression plasmid that targets the DNMT1 gene of human genome.

gRNA and WT SpCas9 co-expression plasmid: pLentiCRISPR v2 DNMT1

Reference Sequence View the backbone

pLentiCRISPR v2 DNMT1

Cat. No.:

MC_0055280

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$99

General

Specification

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Keywords	CRISPR
Gene Targeted	DNMT1
Gene Target Sequence	TTTCCAAACCTCGCACGCCC
Species	H. sapiens (human)
Description	The following gRNA sequence is designed by Feng Zhang’s laboratory at the Broad institute to uniquely target the indicated gene within the human genome. The gRNA sequence is for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. The sgRNA sequence was validated by Feng Zhang’s research.
Vector Backbone	pLentiCRISPR v2
Selectable Marker	Puromycin
Reference	Sanjana N.E., Shalem O., Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods (2014) 11(8): 783-4.

Plasmid Copy	High Copy
Bacterial Resistance	Ampicillin
Growth Strain	Stbl3
Growth Temperature	37°C
Plasmid Size (bp)	13013
Gene/Insert Name	DNMT1
Gene/Insert Sequence	TTTCCAAACCTCGCACGCCC

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The following gRNA sequence is designed by Feng Zhang’s laboratory at the Broad institute to uniquely target the indicated gene within the human genome. The gRNA sequence is for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. The sgRNA sequence was validated by Feng Zhang’s research.

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