pGex MAP3K2 FL K260A plasmid from nicolas reynoird

Sequence by GenScript Sequence by Depositor

pGex MAP3K2 FL K260A

Cat. No.:

MC_0000664

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$65

General

Specification

Comments (0)

Keywords	Expression
Vector Backbone	pGex6.1
Source	Member Deposit
Depositor	nicolas reynoird
Organization	Institute of Advanced Biosciences
Description	bacterial expression
Publication	P. K. Mazur, N. Reynoird, P. Khatri, P. W. Jansen, A. W. Wilkinson, S. Liu, O. Barbash, G. S. Van Aller, M. Huddleston, D. Dhanak, P. J. Tummino, R. G. Kruger, B. A. Garcia, A. J. Butte, M. Vermeulen, J. Sage, and O. Gozani, 'Smyd3 Links Lysine Methylation of Map3k2 to Ras-Driven Cancer', Nature, 510 (2014), 283-7.

Plasmid Copy	High Copy
Bacterial Resistance	Ampicillin
Growth Strain	TOP10
Growth Temperature	37°C
Plasmid Size (bp)	0
Gene/Insert Name	MAP3K2 K260A
Gene/Insert Sequence	ATGGATGATCAGCAAGCTTTGAACTCAATCATGCAAGATTTGGCTGTCCTTCATAAGGCCAGTCGACCAGCATTATCCTTGCAGGAAACCAGAAAAGCAAAATCTTCATCACCAAAAAAACAGAATGATGTCCGAGTCAAATTTGAACATAGAGGAGAAAAAAGAATCCTTCAGTTCCCCAGACCAGTTAAACTGGAAGATCTGAGATCTAAAGCTAAAATTGCCTTTGGACAGTCTATGGATCTACATTATACCAATAACGAGTTGGTAATTCCATTAACTACTCAAGATGACTTGGACAAAGCTGTGGAACTGCTGGATCGTAGTATTCATATGAAGAGCCTCAAGATATTACTTGTAATAAATGGAAGTACACAGGCTACTAATTTAGAACCATTGCCATCACTAGAAGATTTGGATAATACAGTATTTGGAGCAGAGAGGAAAAAACGGCTATCTATAATAGGTCCTACTAGTAGAGATAGAAGTTCTCCTCCCCCAGGTTACATTCCAGATGAATTACACCAGGTTGCCCGGAATGGGTCATTCACTAGTATCAACAGTGAAGGAGAGTTCATTCCAGAGAGCATGGACCAAATGCTGGATCCATTATCTTTAAGCAGCCCTGAAAATTCTGGCTCAGGAAGTTGTCCATCACTTGATAGTCCTTTGGATGGAGAGAGCTATCCAAAATCACGAATGCCTAGGGCTCAGAGCTACCCAGATAATCATCAGGAATTTTCAGACTATGATAACCCTATCTTTGAGAAATTTGGAGCAGGAGGAACATATCCAAGAAGGTATCATGTTTCATATCATCATCAAGAGTATAATGATGGTCGTAAAACTTTTCCAAGAGCTAGAAGGACCCAGGGGACCAGCTTACGGTCTCCTGTGAGTTTCAGTCCTACTGATCATTCCTTAAGCACTAGTAGTGGAAGCAGTATCTTTACCCCAGAGTATGATGATAGTCGAATAAGAAGAAGGGGAAGTGACATAGACAATCCTACTTTGACCGTAATGGACATCAGCCCACCCAGCCGTTCACCTCGAGCTCCGACCAACTGGAGATTGGGCAAACTGCTTGGCCAAGGAGCCTTTGGAAGGGTCTACCTCTGTTATGATGTTGATACAGGAAGAGAATTGGCTGTTAAGCAAGTTCAATTTGACCCCGATAGTCCTGAGACCAGCAAGGAAGTAAATGCACTTGAGTGTGAAATTCAGTTGCTGAAAAACTTGCTACATGAGCGAATTGTTCAGTATTATGGCTGTTTGAGGGATCCCCAGGAAAAAACACTTTCCATATTTATGGAATATATGCCAGGGGGTTCAATTAAGGACCAATTAAAAGCATATGGCGCTCTTACTGAGAATGTGACTAGGAAATACACCCGTCAGATTCTGGAGGGTGTCCATTATTTGCACAGTAATATGATTGTCCATAGAGATATCAAAGGCGCAAATATCCTGCGAGATTCAACAGGCAACGTCAAACTAGGAGATTTTGGGGCCAGCAAACGGCTTCAGACCATCTGTCTCTCAGGGACAGGAATGAAGTCTGTCACGGGCACACCATACTGGATGAGCCCTGAAGTCATCAGTGGAGAAGGCTATGGAAGAAAAGCAGACATCTGGAGTGTTGCATGTACTGTGGTAGAAATGCTAACTGAAAAGCCGCCTTGGGCTGAATTTGAAGCAATGGCTGCCATCTTTAAAATCGCCACTCAGCCAACAAACCCAAAGCTGCCACCTCATGTCTCAGACTATACTCGAGATTTCCTCAAACGGATTTTTGTAGAGGCCAAACTGAGACCTTCAGCTGATGAACTCTTAAGGCACATGTTTGTGCATTATCACTAG

*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.

Customers who viewed this item also viewed:

pRed_Cas9_recA_∆poxb300

CRISPR

pRed_Cas9_∆poxB300

CRISPR

pCas

Constitutive expression of Cas9 together with inducible expression of lambda RED and sgR

pCAGO

CRISPR/Cas9-assisted gRNA-free one-step genome editing

pUC57-2019-nCoV-PC:RdRP

This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR

pUC57-2019-nCoV-S(Original)

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (original sequence)

pUC57-2019-nCoV-S(Human)

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Human expression system)

pUC57-2019-nCoV-N

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) nucleocapsid phosphoprotein (original sequence)

pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)

Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).

2019-nCov_pcDNA3.1(+)-P2A-eGFP

The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.