pCgsgRNA_crtYf plasmid from Sheng Yang

Sequence by GenScript Sequence by Depositor

pCgsgRNA_crtYf

Cat. No.:

MC_0101143

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$65

Edit in GenSmart Design

General

Specification

Comments (0)

Keywords	CRISPR
Vector Backbone	pJYS2_crtYf
Source
Depositor	Sheng Yang
Organization	Center for Excellence in Molecular Plant Sciences (CEMPS), CAS
Description	Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
Publication	De novo engineering of Corynebacterium glutamicum for L-proline production

Plasmid Copy	Unknown
Bacterial Resistance	Spectinomycin
Growth Strain	DH5-Alpha
Growth Temperature	37°C
Plasmid Size (bp)	4214
Gene/Insert Name	sgRNA
Gene/Insert Sequence	actaataacgtaacgtgactggcaagagatatttttaaaacaatgaataggtttacacttactttagttttatggaaatgaaagatcatatcatatataatctagaataaaattaactaaaataattattatctagataaaaaatttagaagccaatgaaatctataaataaactaaattaagtttatttaattaacaactatggatataaaataggtactaatcaaaatagtgaggaggatatatttgaatacatacgaacaaattaataaagtgaaaaaaatacttcggaaacatttaaaaaataaccttattggtacttacatgtttggatcaggagttgagagtggactaaaaccaaatagtgatcttgactttttagtcgtcgtatctgaaccattgacagatcaaagtaaagaaatacttatacaaaaaattagacctatttcaaaaaaaataggagataaaagcaacttacgatatattgaattaacaattattattcagcaagaaatggtaccgtggaatcatcctcccaaacaagaatttatttatggagaatggttacaagagctttatgaacaaggatacattcctcagaaggaattaaattcagatttaaccataatgctttaccaagcaaaacgaaaaaataaaagaatatacggaaattatgacttagaggaattactacctgatattccattttctgatgtgagaagagccattatggattcgtcagaggaattaatagataattatcaggatgatgaaaccaactctatattaactttatgccgtatgattttaactatggacacgggtaaaatcataccaaaagatattgcgggaaatgcagtggctgaatcttctccattagaacatagggagagaattttgttagcagttcgtagttatcttggagagaatattgaatggactaatgaaaatgtaaatttaactataaactatttaaataacagattaaaaaaattataaaaaaattgaaaaaatggtggaaacacttttttcaatttttttgttttattatttaatatttgggaaatattcattctaattggtaatcagattttagaaaacaataaacccttgcatatgatatcgatgtacagatctttttttaaggcagttattggtgcccttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgccgccttctatgaaagccatcaatcctgcctatttgccacgtttaacaaggtagttaagcgttcatttacgaagaaaacacgataagctgcacaaatacctgaaaaagttgaacgccccgtgagcgggaactcacagggcgtcggctaacccccagtcatcagctgggagaaagcactcaagacatgactctagccgatccgcaggacacagtcacagctagcgcgtggaaattgtccgccgatctgttcgacacccaccccgaagctatgcgctgcggctcacgcggctggacggcagaagatcgccgcgaactgctcgctcacctgggacgcgaaagcttccagggcagcaagacaagagatttcgcgagcgcctggattaaaaacccggataccggcgaaacccaaccaaagctctaccgggctggctcaaaagcgctgacgcggtgccagtacgttgcgctgacgcacgcgcaacatgccgcggtgatcgtgcttgacatcgatgtgcccagccaccaggccggcgggaagattgagcacgtaaacccgcaggtctacgcgattttagagaaatgggcacgcctagaaaaagcgccggcttggatcggcgtgaatccgctgagcgggaaatgccagctcatctggctcattgacccggtgtatgccgcagcaggtaaaaccagcccaaatatgcgcctgctggctgcaacgacggaagaaatgactcgtgttttcggcgctgaccaggctttttcgcataggctgagccggtggccgctgcacgtctcagacgatccgacagcctataaatggcactgccagcatgatcgtgtggatcggctggccgacctaatggagattgctcgaacgatgaccggatcacagaagccgaaaaagtacattgagcaggacttttccagcggacgcgcccgcattgaagcggcacaacgcgccaccgcagaagccaaggcgctagcgattttggacgcgagcctgccgagcgccctggacgcgtccggcgacctgatcgacggcgtgcgagtgctctggacaaatccagagcgagcgcgcgacgagaccgcgtttcgccacgcgttgaccgtgggataccagctcaaagctgctggtgagcgcctaaaagatgccaagatcatcgacgcgtatgaagtggcgtacaacgttgcccaggcggtcggtgcagacggccgggagccggatcttcccgccatgcgtgatcgcctgacgatggcgcgtcgtgtgcgcggctacgtggctaaaggccagccagtcgtccctgctcgtcgggtggaaacgcagagcagccgagggcggaaagctctagcgacgatggggcgacggggcgcagctacatcgaatgcacgcagatgggctgacccagaaagtaagtatgcgcaggagacgcgacagcgattagcggaagcaaacaaacgccgagaaatgacaggcgagttgctcgaacttcgcgtcaaaactgcgatcctggatgcccgttctcaatcggttgctgatccctcgactcgtgagcttgcaggcgaactaggtgtcagtgaaaggcgcatccaacaagtcagaaaggcacttggaatggaagctaaacgcggccgtccacgggctgaaaactaataaacgaaacaccgtcagcagaaaacggttcccccctttaggggtcccgtccttgctctggctctcacttgccctcaccctccgctatccacgggctgaaaactaataaacgaaacaccgtcagcagaaaacggttccccccctttagggtgtctcgctcctagctctgatccctccccggttcctccccggcctgatttttaaggggggctcacgctgtcggcagagaacggttccccgccttctgctctggctcttcctcgactccctccccctcaaaaatctcctcgagtctagattgacagctagctcagtcctaggtataatggatccctgcgtttaaacatatttccgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttaagcttcatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatga

*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.

Customers who viewed this item also viewed:

pRed_Cas9_recA_∆poxb300

CRISPR

pRed_Cas9_∆poxB300

CRISPR

pCas

Constitutive expression of Cas9 together with inducible expression of lambda RED and sgR

pCAGO

CRISPR/Cas9-assisted gRNA-free one-step genome editing

pUC57-2019-nCoV-PC:RdRP

This plasmid contains part of 2019-nCoV(SARS-CoV-2) RdRP gene and can be used as positive control for the detection of 2019-nCoV(SARS-CoV-2) by qRT-PCR

pUC57-2019-nCoV-S(Original)

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (original sequence)

pUC57-2019-nCoV-S(Human)

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) surface glycoprotein (Codon optimized for Human expression system)

pUC57-2019-nCoV-N

This plasmid contains the encoding gene of 2019-nCoV(SARS-CoV-2) nucleocapsid phosphoprotein (original sequence)

pcDNA3.1+/C-(K)DYK-ACE2 (NM_021804.2, OHu20260)

Homo sapiens angiotensin I converting enzyme 2 (ACE2), the receptor for 2019-nCoV(SARS-CoV-2).

2019-nCov_pcDNA3.1(+)-P2A-eGFP

The plasmid is used for 2019-nCoV(SARS-CoV-2) surface glycoprotein expression (Codon Optimized for Mouse expression system). The surface glycoprotein is integrated with eGFP protein through P2A, which can be used for flow cytometry antibody screening or immunological research.