asgRNA plasmid plasmid from Friedrich C. Simmel
asgRNA plasmid
Cat. No.:
MC_0009997
Availability:
4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
(1 µg for low-copy plasmid)
Price:
$65
| Keywords | Synthetic biology |
| Vector Backbone | |
| Source | Member Deposit |
| Depositor | Friedrich C. Simmel |
| Organization | Technical University Munich |
| Description | The plasmid encodes anti-sense RNA molecules for the sgRNA sequences from the CRISPRi plasmid. The plasmid contains the anti-sgRNA sequecnces with a pLux promoter, mRFP with a pLac promoter, LuxR and decoy binding sites for the dCas9:sgRNA from the CRISPRi plasmid. |
| Publication | A. Mückl, M. Schwarz-Schilling, K. Fischer, and F. C. Simmel, "Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach", PLOS ONE 13, e0198058 (2018). |
| Plasmid Copy | High Copy |
| Bacterial Resistance | Kanamycin |
| Growth Strain | |
| Growth Temperature | 37°C |
| Plasmid Size (bp) | 4867 |
| Gene/Insert Name | mRFP, LuxR |
*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
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