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asgRNA plasmid
The plasmid encodes anti-sense RNA molecules for the sgRNA sequences from the CRISPRi plasmid. The plasmid contains the anti-sgRNA sequecnces with a pLux promoter, mRFP with a pLac promoter, LuxR and decoy binding sites for the dCas9:sgRNA from the CRISPRi plasmid.
pLentiCRISPR v2 MTHFR
The following gRNA sequence is designed by Feng Zhang’s laboratory at the Broad institute to uniquely target the indicated gene within the human genome. The gRNA sequence is for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. The sgRNA sequence was validated by Feng Zhang’s research.
pHT-XCR2
The plasmid is used for the expression of Cpf1 and λ-Red in B. subtilis,and can replicate in E. coli.