Calmodulin-WT plasmid from Shuo Huang
Calmodulin-WT
Cat. No.:
MC_0102111
Availability:
4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)
(1 µg for low-copy plasmid)
Price:
$65
| Keywords | Expression |
| Vector Backbone | pET-22b(+) |
| Source | |
| Depositor | Shuo Huang |
| Organization | Nanjing University |
| Description | The plasmid Calmodulin-WT is designed for the preparation of wild-type calmodulin (CaM). CaM is a 17-kDa calcium-binding messenger protein that serves as a critical link in the signal transduction pathway between Ca²⁺ and target proteins in eukaryotes. The structures of CaM in the absence of Ca²⁺ (PDB: 1CFC) and in the presence of Ca²⁺ (PDB: 1CLL) have been well-characterized. CaM can be effectively detected by the MspA nanopore, and its different conformers, including the Ca²⁺-free, Ca²⁺-bound, and target peptide-bound states can be unambiguously distinguished (Angewandte Chemie International Edition, 2021, 60(44), 23863-23870.). |
| Publication |
| Plasmid Copy | High Copy |
| Bacterial Resistance | Ampicillin |
| Growth Strain | E. coli BL21(DE3) |
| Growth Temperature | 37°C |
| Plasmid Size (bp) | 5802 |
*This material may be covered by one or more patents, trademarks and/or copy rights owned or controlled by Depositors or any third parties. This material is available to academic and nonprofit organizations for research use only. Please contact MolecularCloud at plasmid@genscript.com and the Depositor directly if you are from industrial institute or attempt for profit application. This material is not intended to be used therapeutic or diagnostic purposed in humans or animals.
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