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N-terminal 10-His SUMO fusion vectors for standard protein expression: scarless removal of the His tag is accomplished using SUMO protease; a SUMO fusion can promote protein folding and solubility
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N-terminal 10-His DsbC TEV-peptide fusion vectors for expression of proteins with one or more disulfide bonds: this is a fusion to the mature form (i.e. the secretion signal peptide has been removed) of E. coli disulfide bond isomerase, which promotes expression and folding of proteins containing multiple disulfide bonds from the cytoplasm; scarless removal of the fusion domain is accomplished using TEV protease