pLentiCRISPR v2 LGR4 is a gRNA and WT SpCas9 co-expression plasmid that targets the LGR4 gene of human genome.

gRNA and WT SpCas9 co-expression plasmid: pLentiCRISPR v2 LGR4

Reference Sequence View the backbone

pLentiCRISPR v2 LGR4

Cat. No.:

MC_0059469

Availability:

4 µg of lyophilized plasmid
(1 µg for low-copy plasmid)

Price:

$99

General

Specification

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Keywords	CRISPR
Gene Targeted	LGR4
Gene Target Sequence	GGAAGGGCTTTAATAGCCTG
Species	H. sapiens (human)
Description	The following gRNA sequence is designed by Feng Zhang’s laboratory at the Broad institute to uniquely target the indicated gene within the human genome. The gRNA sequence is for use with WT SpCas9, or as crRNA for use with WT SpCas9 protein, to introduce a DSB for genome editing. The sgRNA sequence was validated by Feng Zhang’s research.
Vector Backbone	pLentiCRISPR v2
Selectable Marker	Puromycin
Reference	Sanjana N.E., Shalem O., Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods (2014) 11(8): 783-4.

Plasmid Copy	High Copy
Bacterial Resistance	Ampicillin
Growth Strain	Stbl3
Growth Temperature	37°C
Plasmid Size (bp)	13013
Gene/Insert Name	LGR4
Gene/Insert Sequence	GGAAGGGCTTTAATAGCCTG

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