H-Leu-Ser-p-nitro-Phe-Nle-Ala-Leu-OMe

Description:

Standard substrate for chymosin activity determination. This peptide is also a substrate for pepsin, penicillopepsin, and gastricsin.

Sequence:

LS-Nph-Nle-AL -OMe

General

References

Comments (0)

Name	H-Leu-Ser-p-nitro-Phe-Nle-Ala-Leu-OMe
Category	Enzyme Substrates and Inhibitors
One Letter Code	LS-Nph-Nle-AL -OMe
Three Letter Code	{Leu}{Ser}{p}{nitro}{Phe}{Nle}{Ala}{Leu}-OMe
Molecular Weight	721.850
Application

Malone, A. S., et al. "High pressure effects on proteolytic and glycolytic enzymes involved in cheese manufacturing." Journal of Dairy Science 86.4 (2003): 1139-1146.

Williams, Mark G., et al. "Mutagenesis, biochemical characterization and X-ray structural analysis of point mutants of bovine chymosin." Protein engineering 10.9 (1997): 991-997.

Cho, Yong-Kweon, and Dexter B. Northrop. "Transpeptidation by porcine pepsin catalyzed by a noncovalent intermediate unique to its iso-mechanism." Journal of Biological Chemistry 273.38 (1998): 24305-24308.