Z-Arg-Arg-2-Naphthylamine

Description:

Specific substrate of maximal sensitivity for cathepsin B, free from interference by cathepsin H. Stock solutions of this substrate are best prepared in DMSO.

Sequence:

CBZ-RR-2-Naphthylamine
  • General
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  • Name Z-Arg-Arg-2-Naphthylamine
    Category Enzyme Substrates and Inhibitors
    One Letter Code CBZ-RR-2-Naphthylamine
    Three Letter Code CBZ-{Arg}{Arg}-2-Naphthylamine
    Molecular Weight 589.700
    Application
    Knight, C. Graham. "Human cathepsin B. Application of the substrate N-benzyloxycarbonyl-L-arginyl-L-arginine 2-naphthylamide to a study of the inhibition by leupeptin." Biochemical Journal 189.3 (1980): 447-453.
    Willenbrock, Frances, and Keith Brocklehurst. "A general framework of cysteine-proteinase mechanism deduced from studies on enzymes with structurally different analogous catalytic-site residues Asp-158 and− 161 (papain and actinidin), Gly-196 (cathepsin B) and Asn-165 (cathepsin H). Kinetic studies up to pH 8 of the hydrolysis of N-α-benzyloxycarbonyl-l-arginyl-l-arginine 2-naphthylamide catalysed by cathepsin B and of l-arginine 2-naphthylamide catalysed by cathepsin H." Biochemical Journal 227.2 (1985): 521-528.
    Kirschke, H., et al. "Action of rat liver cathepsin L on collagen and other substrates." Biochemical Journal 201.2 (1982): 367-372.
    Knight, C. Graham. "Human cathepsin B. Application of the substrate N-benzyloxycarbonyl-L-arginyl-L-arginine 2-naphthylamide to a study of the inhibition by leupeptin." Biochemical Journal 189.3 (1980): 447-453.
    Barrett, Alan J. "An improved color reagent for use in Barrett's assay of cathepsin B." Analytical biochemistry 76.1 (1976): 374-376.
    McDonald, J. Ken, and Stanley Ellis. "On the substrate specificity of cathepsins B1 and B2 including a new fluorogenic substrate for cathepsin B1." Life sciences 17.8 (1975): 1269-1276.
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